Characterization of human FCRL4-positive B cells

Characterization of human FCRL4-positive B cells

FCRL4 is an immunoregulatory receptor that belongs to the Fc receptor-like (FCRL) family. In healthy individuals, FCRL4 is specifically expressed by memory B cells (MBCs) localized in sub-epithelial regions of lymphoid tissues. Expansion of FCRL4+ B cells has been observed in blood and other tissues in various infectious and autoimmune disorders. Currently, the mechanisms involved in pathologic...

Antigenic phenotype and functional characterization of human tonsil B cells.

Human tonsil cells were labeled with anti-leu-16, a monoclonal antibody (MoAb) that recognizes the CD20 antigen and that is specific for B cells. Two populations of B cells were identified by flow cytometry on the basis of antigen density. One labeled brightly with anti-Leu-16, and the other labeled at a level comparable to blood B cells. These two populations were characterized with a panel of...

Identification and characterization of circulating human transitional B cells.

Murine B-cell development begins in bone marrow and results in the generation of immature transitional B cells that transit to the spleen to complete their maturation. It remains unclear whether the same developmental pathway takes place in humans. Using markers characteristic of human bone marrow immature B cells, we have identified a population of circulating human B cells with a phenotype mo...

Immunoglobulin characteristics and RNAseq data of FcRL4+ B cells sorted from synovial fluid and tissue of patients with rheumatoid arthritis

This manuscript is a companion paper to Amara et al. [1]. Data shown here include detailed clinical characteristics from anonymized patients, the Ig subclass data generated from B cells sorted from four individual patients, tables detailing variable gene region sequences from sorted cells linked to the patient information and the sequence yields from individual patients. Furthermore a URL link ...

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells